Maintenance of brazilian biodiversity by germplasm bank / Manutenção da biodiversidade brasileira através de bancos de germoplasma

Currently the importance of using alternative strategies for biodiversity conservation is emphasized and since the establishment of germplasm bank is an alternative to the conservation of endangered species. This is a technique of great importance for the maintenance of Brazilian fauna. Since the early70'sthere was a growing concern about the need to preserve essential genetic resources for food and agriculture, mainly for conservation of genetic material from farm animals. Thus was created the Brasilia Zoo, in July 2010, the first Germplasm Bank of Wild Animals in Latin America, as an alternative strategy for the conservation of threatened or endangered species, using both gametes and somatic cells and stem cells. Then we argue to create new banks or research networks among different regions with aimed to tissue preservation.

Atualmente, a importância do uso de estratégias alternativas para a preservação da biodiversidade é ressaltada e, visto que a criação de bancos de germoplasma é uma alternativa para a conservação de espécies ameaçadas, esta é uma técnica de suma importância para a manutenção da fauna brasileira. Desde o começo da década de 70 houve uma crescente preocupação sobre a necessidade de se preservar recursos genéticos essenciais para alimentação e agricultura, voltados principalmente, para a conservação de material genético de animais de produção. Deste modo, foi criado pelo Jardim Zoológico de Brasília, em julho de 2010, o primeiro Banco de Germoplasma de Animais Selvagens da América Latina, como uma estratégia alternativa para a conservação de espécies ameaçadas ou em perigo de extinção, utilizando tanto gametas como células somáticas e células-tronco. Com isto ponderamos na criação de novos bancos ou redes de pesquisa inter-regionais que foquem nesta preservação tecidual.

Reproducibility of nuclear morphometry parameters from cytologic smears of canine cutaneous mast cell tumors--intra- and interobserver variability.

BACKGROUND: Qualitative and/or semiquantitative histopathologic grading has been the method of choice to predict the biologic behavior of mast cell tumors (MCT). Recently, computer-assisted nuclear morphometric analysis of histologic or cytologic specimens has been shown to correlate with histopathologic grading of MCT. However, it is still not clear what the reproducibility and variability of these computer-assisted measurements are. OBJECTIVES: The objective of this study was to estimate the intra- and interobserver variability in computer-assisted morphometric analysis of nuclear parameters in fine-needle cytologic smears of canine cutaneous MCT. MATERIALS AND METHODS: Measurements were performed by 2 observers with basic training on the recognition of neoplastic cells and operating the image analysis software. The analyses were performed twice at 6 months apart. RESULTS: No significant effects were detected for observer, time, and the interaction observer-time. CONCLUSIONS: Nuclear morphometric analysis of mast cell tumors using cytologic smears is a reproducible method for grading of MCT.